Sputum for Cytology

Test Name:   Sputum for Cytology
Alternate Name (s):    
Laboratory Module:   Cytology
Ordering Mnemonic:   All specimens must be accompanied by a completed manual or Meditech generated cyto-pathology non-gynecological requisition. All mandatory fields must be satisfied in order to complete the request. In the case of computer downtime use the standard cytopathology non-gynecological requisition.
Specimen Type:   

Sputum (Expectorated or Suctioned)

Collection Container:   Collect in a sterile container
Container Information
Sterile Container
Collection Information:  

I.1.All specimens must be accompanied by a completed manual or Meditech generated cyto-pathology non-gynecological requisition. All mandatory fields must be satisfied in order to complete the request. In the case of computer downtime use the standard cytopathology non-gynecological requisition.

The following information is required on the requisition
(a) Patient identifiers (addressographs may be used)

      (i) patient's first and last name
      (ii) hospital unique number
      (iii) date of birth
      (iv) Ontario Health Insurance Number with version code.
(b) Submitting area identifiers: Hospital site (BGH or The Willett) and the submitting department (Emergency, Ambulatory Care etc.).
(c) Submitting physician's name.
(d) Date of specimen collection
(e) Pre- and post-operative presumptive diagnosis.
(f) A brief clinical history, relevant pathological and radiological findings.
(g) Specimen information
     (i) specimen type/source
     (ii) specimen site
    
I.2. If received, the specimen container must be labeled. Addressograph labels are preferred, otherwise the following information must be provided, clearly written in ink:
(a) Patient identifiers -
     (i) patient's full name
     (ii) hospital unique number
     (iii) and at least one other unique additional identifier (ie. date of birth, OHIN).
(b) Date of specimen collection.
(c) Specimen type and site, as it is written on the Cytology Requisition form.
This information must be recorded on the side of the specimen container and not the lid.
If a specimen is known or suspected to contain unique or extreme biohazard (e.g. CJD) the container shall be so marked.

I.3.Place labeled slides with the patients name and specimen type, in a cardboard slide holder and drying and attach an addressograph label on the outside of the holder.

I.4. Place specimen containers in a biohazard bag (if applicable). The requisition should be placed in the outer pouch of the biohazard bag.

I.5. Transport specimen to the Triage area of the laboratory as soon as possible

I.6. Inform the triage staff that you have delivered a specimen.

 

Collection of Sputum

Fresh, deep cough, early morning specimens are collected prior to eating, drinking, or brushings teeth. Cough deep from the lungs and spit the mucoid material into a clean sterile container.

Continue coughing until an adequate sample is produced, then cap the container tightly and write the patient information on it (as indicated above).

It is extremely important that the material submitted is from the deep lung cough and not saliva from the nose or throat.

Return the labeled container with the specimen to the laboratory. If a delay in transport ensure the specimen is refrigerated to prevent the cellular detail from degenerating.

This test is repeated 3 times on different days.

Note: If the specimen is a post-bronchoscopy specimen this needs to be indicated since the instrument can cause cellular changes and cause diagnostic difficulties.

Test Schedule:  

Monday to Friday prior to 1:00 pm

Turnaround Time:   Routine

1 week

Stat / Urgent

2 days

Reference Interval:  

 

Critical Values:    
Lab Process Notes :   Cytology fixative should be added if processing delay is greater than 72 hours.
Storage & Transport:   Specimens should be stored in the refrigerator
Test Referred to :    BGH, On site
 
Designed By  Blueprint Agencies