crytococcus Serology

Cryptococcus Antigen

Test Name:  

Cryptococcus Antigen

Alternate Name (s):  

 

Laboratory Module:  

Microbiology

Ordering Mnemonic:  

CRYPTO CSF

Specimen Type:   

Cerebral Spinal Fluid

Collection Container:  

Sterile CSF conical tubes

csf.jpg

Collection Information:  

Tube 1- Glucose and protein

Tube 2- Microbiology (other tests)

Tube 3- Cell Count (Microbiology is x4 tubes collected)

Tube 4- Cell Count

The last tube collected will be processed for the cell count

 

Lumbar Puncture

Clean the puncture site with antiseptic solution and alcohol before needle insertion. Insert a needle with stylet at the L3-L4, L4-L5 or L5-LS interspace. When subarachnoid space is reached, remove stylet and spinal fluid will appear in the needle hub. Slowly drain CSF into sterile tubes.

Shunt Fluid

Clean the reservoir site with antiseptic solution and alcohol. Collect fluid through the reservoir unit and place it in a sterile tube.
Indicate method of collection on requisition.

Suggested Volumes:

Routine Culture (C&S) - 2 mL

Crytococcus Antigen- 1 mL

Fungus Culture - 2 mL

Mycobacterium Culture - 2 mL

Virus Culture - 2 mL

Test Schedule:  

Monday to Friday

Turnaround Time:  

Routine

Preliminary: 48 hours

Final: 72 hours

Stat / Urgent

Preliminary: 24 hours

Final: 48 hours

Reference Interval:  

 

Critical Values:  

 

Lab Process Notes :  

Note: always send the most turbid tube to Microbiology.

Note: Specimen description and total quantity will be recorded for the CSF

The supernatant/extra specimen will be frozen in the -70'C freezer for approximately 6 months.

Storage & Transport:  

The specimen should be delivered to the laboratory STAT, with no delay and should not be transported through the automated delivery system.

CSF- If after hours and non call back has been initiated: if sufficient specimen either the supernatant or any extra containers can be stored in STAT container in the refrigerator.

Serum- store in the refrigerator

CSF for culture is never to be stored in the refrigerator.

Test Referred to :   

Public Health Laboratories, Hamilton

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Updated August 13, 2013 ll

 
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