(APCR) Activated Protein C Resistance

Activated Protein C Resistance (Screen for Factor 5 Leiden)

Test Name:   Activated Protein C Resistance
Alternate Name (s):    Factor 5 Leiden
Laboratory Module:   LAB
Ordering Mnemonic:   APCR
Specimen Type:   

Citrated Plasma and EDTA whole blood 

Collection Container:  

Blue- 5.0 mL for APCR

Lavender 5.0 mL for Factor 5 Leiden

Container Information
Blue Tubelavender_tube.jpg
Collection Information:   Patient should not be on anticoagulant therapy for one week prior to collection.

Double spin plasma. Ship frozen on dry ice.

 

* LT Blue
Specimen Handling:
ALWAYS partially fill a DISCARD TUBE first.

Then collect blood in a LIGHT BLUE top tube (citrated).
Collect a FULL TUBE. Mix thoroughly by gentle inversion.
Dispose the DISCARD TUBE into the biohazardous container.

IMMEDIATELY centrifuge for 15 minutes at 3500 rpm.
Carefully pipette all PLATELET POOR plasma,
starting from the top of the plasma, into a clear labelled centrifuge tube taking care NOT TO DISTURB the buffy coat layer
(white layer of platelets and white cells directly above the red cells).
Cap centrifuge tube.

IMMEDIATELY centrifuge this plasma for another 15 minutes
at 3500 rpm. Pipette the PLATELET POOR plasma, starting from the top of the plasma into one (1) labelled clear 12x75 mm aliquot tube taking care NOT TO DISTURB the BOTTOM of the tube. DO NOT TRANSFER ANY CELLS.
Cap tightly and FREEZE IMMEDIATELY.
Storage: Frozen (-20°C)

Transportation: Frozen. Specimens

FACTOR 5 Leiden:

Sample Requirements:
*Lavender 
Specimen Handling: 
Collect blood. Mix thoroughly by gentle inversion.

To be received within 5 days.

Storage: Room temperature (18-27°C)

Transportation: Room Temperature

Test Schedule:  

 

Turnaround Time:   Routine

20 days

Stat / Urgent
Reference Interval:  

 

Critical Values:    
Lab Process Notes :    Factor 5 leiden will be performed if APCR is abnormal
Storage & Transport:  

Plasma - Ship frozen on dry ice. Store -40'C.

EDTA whole blood ship at ambient temperature

Test Referred to :    Reference Lab

                 

Updated2017.04.17 ll

 
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